mouse anti acj6 monoclonal  (Developmental Studies Hybridoma Bank)

Journal: eLife

Article Title: A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord

doi: 10.7554/eLife.54074

Figure Lengend Snippet: ( A ) Visualization of acj6 expressing cells in the 5 day old adult VNC. Maximal coronal projection, and hemi-maximal sagittal projectionof acj6 GAL4 driving expression of UAS-WM , enabling the visualization of cell nuclei (nRFP; green) and neuronal morphology (mGFP; magenta). Neuropil is counterstained (Brp; blue). A, Anterior, P, posterior, D, dorsal; scale bars = 50 μm. ( B ) t-SNE plot of acj6 expression in the VNC. Predicted hemilineage 8B, 9B, and 23B, based on known markers (shown below) are highlighted. ( C ) t-SNE plots of predicted hemilineage 23B, showing expression of markers acj6 , unc-4 , and VAChT . ( D ) t-SNE plots of predicted hemilineage 23B, showing expression of novel sub-type markers kn and twz . ( B–D ) Expression shown in black, intensity is proportional to the log-normalized expression levels. ( E ) Maximal coronal view (left) and hemi-maximal sagittal view (right) of Acj6 expression (green) in the 5 day old adult VNC. The posterior prothoracic hemilineages 23B (dashed white box) and the anterior mesothoracic 8B and 9B (dashed yellow box) are marked. A, Anterior, P, posterior, D, dorsal; Neuropil is counterstained (Cad-N; blue) scale bars = 50 μm. ( F ) Close-up maximal coronal views of dorsolateral hemilineage 23B showing the co-expression of kn GAL4 and twz GAL4 driven UAS-Stinger expression (nGFP; magenta) with Acj6 (green). Co-positive cells (white) are indicated with arrow heads. Lim3 GAL4 driven expression did not colocalize with Acj6 in hemilineage 23B. GOI = gene of interest, scale bars = 10 μm. Figure 5—source data 1. Cell counts of novel sub-lineage marker co-expression in the VNC. Cell counts of Acj6-positive and Acj6/GOI co-positive cells in prothoracic hemilineage 23B and mesothoracic hemilineages 8B and 9B. Values are for a single side of a single individual (along with means, standard deviations, and standard errors).

Article Snippet: Primary antibodies used were: mouse mAb nC82 (1:50), mouse anti-AbdA (1:100, C-11), mouse anti-AbdB (1:10, 1A2E9-s), mouse anti-Acj6-s (1:20), mouse anti-AntP (1:20, 8C11-s), mouse anti-Ubx (1:20, FP3.38-s), rat anti-CadN (1:30, DN-Ex #8;) from DSHB, Univ. of Iowa.

Techniques: Expressing, Marker

Journal: eLife

Article Title: A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord

doi: 10.7554/eLife.54074

Figure Lengend Snippet: Maximal projections showing co-expression of acj6 GAL4 driven expression of UAS-Stinger (nGFP; green) and endogenous Acj6 protein expression (anti-Acj6, magenta) in the 5 day old adult VNC. Merged expression shown on right in white. A, Anterior, P, posterior; scale bars = 50 μm.

Article Snippet: Primary antibodies used were: mouse mAb nC82 (1:50), mouse anti-AbdA (1:100, C-11), mouse anti-AbdB (1:10, 1A2E9-s), mouse anti-Acj6-s (1:20), mouse anti-AntP (1:20, 8C11-s), mouse anti-Ubx (1:20, FP3.38-s), rat anti-CadN (1:30, DN-Ex #8;) from DSHB, Univ. of Iowa.

Techniques: Expressing

Journal: eLife

Article Title: A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord

doi: 10.7554/eLife.54074

Figure Lengend Snippet: ( A ) t-SNE plots of Lim3 ( A ), kn ( B ) and twz ( C ) expression in the VNC. Expression shown in black, intensity is proportional to the log-normalized expression levels. ( B ) Maximal projections showing co-expression of Acj6 (anti-Acj6; green) with Lim3 GAL4 , kn GAL4 , and twz GAL4 driven expression of UAS-Stinger (nGFP; magenta) in the 5 day adult VNC; coronal (left) and sagittal (right) views. A, Anterior, P, posterior, D, dorsal; Neuropil is counterstained (CadN; blue); scale bars = 50 μm.

Article Snippet: Primary antibodies used were: mouse mAb nC82 (1:50), mouse anti-AbdA (1:100, C-11), mouse anti-AbdB (1:10, 1A2E9-s), mouse anti-Acj6-s (1:20), mouse anti-AntP (1:20, 8C11-s), mouse anti-Ubx (1:20, FP3.38-s), rat anti-CadN (1:30, DN-Ex #8;) from DSHB, Univ. of Iowa.

Techniques: Expressing

Journal: eLife

Article Title: A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord

doi: 10.7554/eLife.54074

Figure Lengend Snippet: ( A ) Single coronal slices of hemilineage 23B moving from dorsal to ventral side showing co-expression of Acj6 (anti-Acj6; green) with kn GAL4 , and twz GAL4 driven expression of UAS-Stinger (nGFP; magenta), and lack of co-expression of Lim3 GAL4 , in the 5 day adult VNC (GOI = gene of interest; arrow heads indicate co-positive cells; scale bars = 10 μm). ( B ) Single coronal slices of hemilineages 8B and 9B moving from dorsal to ventral side showing co-expression of Acj6 (anti-Acj6; green) with Lim3 GAL4 driven expression of UAS-Stinger (nGFP; magenta), in the 5 day adult VNC (GOI = gene of interest; arrow heads indicate co-positive cells; scale bars = 10 μm).

Article Snippet: Primary antibodies used were: mouse mAb nC82 (1:50), mouse anti-AbdA (1:100, C-11), mouse anti-AbdB (1:10, 1A2E9-s), mouse anti-Acj6-s (1:20), mouse anti-AntP (1:20, 8C11-s), mouse anti-Ubx (1:20, FP3.38-s), rat anti-CadN (1:30, DN-Ex #8;) from DSHB, Univ. of Iowa.

Techniques: Expressing